| 1 |  Lina Marcela Hernandez Gomez (COL) | | 2:26:13 |
| 2 |  Paola Andrea Muñoz Grandon (CHI) | | s.t. |
| 3 |  Teniel Campbell (TRI) | Team Bikeexchange | s.t. |
| 4 |  Lizbeth Yareli Salazar Vázquez (MEX) | A.r. Monex Women's Pro Cycling Team | s.t. |
| 5 | Katherin Steisy Montoya Zuluaga (COL) | | s.t. |
| 6 |  Amber Joseph (BAR) | | s.t. |
| 7 | Scarlet Johanna Cortes Ugarte (CHI) | | s.t. |
| 8 |  Wellynda Regisleyne dos Santos Rodrigues (BRA) | | s.t. |
| 9 | Yana Camargo (BRA) | | s.t. |
| 10 | Erika Milena Botero Lopez (COL) | | s.t. |
| 11 |  Maria José Vargas Barrientos (CRC) | A.r. Monex Women's Pro Cycling Team | s.t. |
| 12 |  Ludisnelis Fleitas Quesada (CUB) | | s.t. |
| 13 |  Angie Sabrina Gonzalez Garcia (VEN) | | s.t. |
| 14 | Milagro Consuelo Mena Solano (CRC) | | s.t. |
| 15 | Katiusca Garcia (VEN) | | s.t. |
| 16 | Marlies Mejías Garcia (CUB) | | s.t. |
| 17 | Ana Vitória Gouvea Vieira Almeida Magalhães (BRA) | | s.t. |
| 18 | Maria Paula Latriglia Alarcon (COL) | | s.t. |
| 19 |  Fabiana Andrea Granizal (URU) | | s.t. |
| 20 | Ana Teresa Casas Bonilla (MEX) | | s.t. |
| 21 | Jeny Lorena Colmenares Colmenares (COL) | | s.t. |
| 22 | Wilmarys Pacheco (VEN) | | s.t. |
| 23 | Romina Hinojosa Cruz (MEX) | | s.t. |
| 24 | Marcia Fernandes Silva (BRA) | | s.t. |
| 25 |  Brenda Mercedes Rodriguez (DOM) | | s.t. |
| 26 | Yudelmis Dominguez Masague (CUB) | | s.t. |
| 27 | Paula Constanza Villabalon Sanchez (CHI) | | s.t. |
| 28 |  Leslye Alexandra Ojeda Rivera (ECU) | | s.t. |
| 29 | Dayana Lizbeth Aguilar Garcia (ECU) | | s.t. |
| 30 |  Cristina Michelle Mata Pitti (PAN) | | s.t. |
| 31 | Karla Vallejos Puchi (CHI) | | s.t. |
| 32 | Aranza Valentina Villalon Sanchez (CHI) | | s.t. |
| 33 | Susan Camacho Fallas (CRC) | | s.t. |
| 34 |  Caitlin Conyers (BER) | | s.t. |
| 35 | Wendy Ducreux (PAN) | | 0:09 |
| 36 | Juana Isidra Fernandez Veras (DOM) | | 0:17 |
| 37 | Stephany Contreras Fernandez (DOM) | | 0:19 |
| 38 | Maria Antonieta Gaxiola Gonzalez (MEX) | | s.t. |
| 39 |  Kaya Cattouse (BLZ) | | 0:22 |
| 40 | Sharon Ramirez Moya (CRC) | | s.t. |
| 41 | Gabriella Tejada Batista (DOM) | | s.t. |
| 42 | Jeydy Pradera Bernal (CUB) | | s.t. |
| 43 | Serika Mitchell Guluma Ortiz (COL) | | s.t. |
| 44 |  Nicole Erato (SXM) | | 0:27 |
| 45 | Aylena De Las Mercedes Quevedo Alvarez (CUB) | | s.t. |
| 46 | Tamires Fanny Radatz (BRA) | | 0:30 |
| 47 | Daniela Alejandra Jimenez Torres (ECU) | | s.t. |
| 48 | Savka Hernandez Levican (CHI) | | 0:36 |
| 49 | Flor Georgina Espiritusanto Estevez (DOM) | | 0:48 |
| 50 | Maria Andreina Daza Santana (VEN) | | 1:05 |
| 51 | Victoria Velasco Fuentes (MEX) | | 3:05 |
| 52 | Maria Teresa Mirabal Cruz (DOM) | | 7:04 |
| 53 |  Romina Maribel Medrano Ilizarbe (PER) | | 8:26 |
| 54 |  Bridggett Lucer Rodriguez Rodriguez (GUA) | | 13:09 |
| 55 |  Micaela Sarabia Ricaldez (BOL) | | s.t. |
| 56 | Abigail Sarabia Ricaldez (BOL) | | 13:11 |
| 57 | Fátima Leonardo Cristales (GUA) | | s.t. |
| DNF | Nikole Pamela Narvaez Pita (ECU) | | - |
| DNF | Karen Duberli Fuentes Orozco (GUA) | | - |
| DNF |  Llori Sharpe (JAM) | | - |
| DNF | Veronica Sofia Abreu Alvarado (VEN) | | - |
| DNF | Paola Belén Silva Wynants (URU) | | - |
| DNF | Angie Paola Bogantes Ruiz (CRC) | | - |
| DNF |  Antinece Simmons (BAH) | | - |
| DNF | Evelyn Diaz Matos (CUB) | | - |
| DNF | Amanda Kunkel (BRA) | | - |
| DNF | Romina Maribel Medrano Ilizarbe (PER) | | - |
| DNF | Diana Solano Rojas (CRC) | | - |
| DNS | Susan Piscione (SXM) | | - |
| DNS |  Lisa Groothuesheidkamp (AHO) | | - |
| DNS | Miryan Maritza Nuñez Padilla (ECU) | | - |
| DNS | Marla Albury (BAH) | | - |
