| 1 |  Skylar Schneider (USA) | | 2:48:08 |
| 2 |  Alison Jackson (CAN) | EF Education - TIBCO - SVB | s.t. |
| 3 |  Catalina Anais Soto Campos (CHI) | Bizkaia - Durango | s.t. |
| 4 |  Andrea Álzate Gomez (COL) | Eneicat - Cmteam - Seguros Deportivos | s.t. |
| 5 |  Ana Vitória Gouvea Vieira Almeida Magalhães (BRA) | Bizkaia - Durango | s.t. |
| 6 | Diana Carolina Peñuela Martinez (COL) | Dna Pro Cycling Team | s.t. |
| 7 | Aranza Valentina Villalon Sanchez (CHI) | Eneicat - Cmteam - Seguros Deportivos | s.t. |
| 8 |  Andrea Ramírez Fregoso (MEX) | Bizkaia - Durango | s.t. |
| 9 | Talita Da Luz De Oliveira (BRA) | | s.t. |
| 10 |  Milagro Consuelo Mena Solano (CRC) | | s.t. |
| 11 | Lina Marcela Hernandez Gomez (COL) | | s.t. |
| 12 |  Claudia Baró Arango (CUB) | | s.t. |
| 13 |  Mariana Garcia Britos (URU) | | s.t. |
| 14 |  Flor Georgina Espiritusanto Estevez (DOM) | | s.t. |
| 15 | Daymelin Perez Quezada (CUB) | | s.t. |
| 16 |  Sofia Martelli (ARG) | | s.t. |
| 17 |  Esther Jessenia Galarza Muñoz (ECU) | | s.t. |
| 18 | Patricia Dolomi Vázquez Cervantes (MEX) | | s.t. |
| 19 |  Paula Guillén (GUA) | | s.t. |
| 20 | Maria Carolina Flores Garcia (MEX) | | s.t. |
| 21 |  Wendy Ducreux (PAN) | | s.t. |
| 22 | Yendry Dixiana Quesada Paniagua (CRC) | | s.t. |
| 23 | Gabriela Alarcon (CHI) | | s.t. |
| 24 | Stefania Sanchez Zapata (COL) | | s.t. |
| 25 | Anabel María Fernanda Yapura Plaza (ARG) | | s.t. |
| 26 | Laura Daniela Rojas Capera (COL) | | 0:03 |
| 27 | Victoria Alejandra Martinez Retamal (CHI) | | s.t. |
| 28 |  Teniel Campbell (TRI) | | s.t. |
| 29 |  Linda Daniela Menendez (HON) | | s.t. |
| 30 | Miryan Maritza Nuñez Padilla (ECU) | Massi - Tactic | s.t. |
| 31 |  Agua Marina Espínola Salinas (PAR) | | s.t. |
| 32 | Romina Hinojosa Cruz (MEX) | | s.t. |
| 33 | Diandra Ramirez Gonzalez (CRC) | | s.t. |
| 34 | Emilie Fortin (CAN) | | s.t. |
| 35 | Aylena De Las Mercedes Quevedo Alvarez (CUB) | | s.t. |
| 36 | Zulianys Fernandez (PAN) | | s.t. |
| 37 | Cavaglieri Aline Fátima Mariga (BRA) | | s.t. |
| 38 | Evelyn Diaz Matos (CUB) | | s.t. |
| 39 | Maria Fernanda Sanchez Vargas (CRC) | | s.t. |
| 40 | Barbara Fiorela Malaspina (ARG) | | s.t. |
| 41 |  Caitlin Conyers (BER) | | s.t. |
| 42 | Johanna Noelia Bracco Olmedo (URU) | | s.t. |
| 43 | Taise Maiara Benato (BRA) | | s.t. |
| 44 | María Catalina Gómez Quiza (COL) | | s.t. |
| 45 |  Sauking Raquel Shi Pimentel (SAL) | | s.t. |
| 46 | Karla Vallejos Puchi (CHI) | | 0:09 |
| 47 | Lina Mabel Rojas Zapata (COL) | | 0:10 |
| 48 | Lauren Stephens (USA) | | 0:13 |
| 49 | Natalia Navarro Cerdas (CRC) | | 0:19 |
| 50 | Amber Neben (USA) | | 0:25 |
| 51 | Naomi Monserrat Langarica Resendiz (MEX) | | 0:27 |
| 52 | Kimberly Juliette Wing (PAN) | | 0:32 |
| 53 | Gabriella Arnold (BER) | | 2:33 |
| 54 | Paula Constanza Villabalon Sanchez (CHI) | | 2:54 |
| 55 | Karen Elisa Amaya (HON) | | 3:36 |
| 56 | Bridggett Lucer Rodriguez Rodriguez (GUA) | | 4:14 |
| 57 | Julieta Benedetti Venturella (ARG) | | 4:22 |
| 58 | Tamires Fanny Radatz (BRA) | | 5:09 |
| 59 | Antonella Leonardi (ARG) | | 6:28 |
| 60 |  Erialis Otero Otero (PUR) | | 8:03 |
| 61 | Madelline Andrea Mendoza Ramos (SAL) | | s.t. |
| 62 |  Abigail Sarabia Ricaldez (BOL) | | 8:06 |
| 63 | Alondra Granados (CRC) | | 8:14 |
| 64 | Sara Nicole Torrico Ortiz (BOL) | | 20:43 |
| 65 | Stephany Contreras Fernandez (DOM) | | s.t. |
| 66 | Valeska Polet Gomez Fuentes (GUA) | | 20:48 |
| 67 | Luciana Wynants Castrillon (URU) | | 21:48 |
| 68 | Ninoska Gissel Andino Portillo (HON) | | s.t. |
| DNF | Belkys Montenegro (PAN) | | - |
| DNF | Thelma Rivera (PUR) | | - |
| DNF | Ana Paula Polegatch (BRA) | | - |
| DNF | Dulce Ajpacajá Tax (GUA) | | - |
| DNF | Kaia Schmid (USA) | Human Powered Health | - |
| DNF | Maraya Lopez (PAN) | | - |
| DNF | Madelin Canela Heredia (DOM) | | - |
| DNF | Annibel Emilia Prieto Ostia de Godoy (PAN) | | - |
| DNF | Marisol Tellado (PUR) | | - |
| DNF | Caileigh Filmer (CAN) | | - |
| DNF | Ana Vivar Torres (ECU) | | - |
| DNF |  Diana Mercedes Marin Fernandez (NIC) | | - |
| DNS |  Lilibeth de Carmen Chacón Garcia (VEN) | | - |
| DNS |  Jurvin Gonzalez (ARU) | | - |
| DNS |  Llori Sharpe (JAM) | | - |
