| 1 |  Brenda Andrea Santoyo Perez (MEX) | | 3:08:00 |
| 2 |  Edith Guillen Chavarria (CRC) | | s.t. |
| 3 | Marcela Rubiano Garces (CRC) | | s.t. |
| 4 |  Flávia Oliveira (BRA) | Health Mate - Cyclelive | s.t. |
| 5 |  Jazmín Gabriela Soto Lopez (GUA) | | s.t. |
| 6 |  Angie Gómez (HON) | | s.t. |
| 7 |  Leidy Natalia Muñoz Ruiz (COL) | | s.t. |
| 8 | Julith Fabiola Arroyo Salas (CRC) | | s.t. |
| 9 | Florida Isabel De León Gonzalez (GUA) | | s.t. |
| 10 | Sindra Viviana Narvaez Rodriguez (COL) | | s.t. |
| 11 | Karol Julieth Moreno Avella (COL) | | s.t. |
| 12 | Yolanda Valderrama Henao (COL) | | s.t. |
| 13 | Lorena Maria Vargas Villamil (COL) | | s.t. |
| 14 |  Marisol Tellado (PUR) | | s.t. |
| 15 | Jennifer Morales Milanes (CRC) | | s.t. |
| 16 | Jeimy Lorena Beltrán Garcia (COL) | | s.t. |
| 17 | Jeniffer Alejandra Medellin Rodriguez (COL) | | s.t. |
| 18 | Andrea Ramírez Fregoso (MEX) | Swapit Agolico | s.t. |
| 19 | Lizbeth Ureño Armas (MEX) | | s.t. |
| 20 | Tatiana Maria Romero Castillo (CRC) | | s.t. |
| 21 | Marcela Elizabeth Prieto Castañeda (MEX) | Swapit Agolico | s.t. |
| 22 | Valentina Blandon Ramirez (COL) | | s.t. |
| 23 | Blanca Liliana Moreno Canchon (COL) | Astana Women's | s.t. |
| 24 | Luisa Fernanda Motavita Duarte (COL) | | s.t. |
| 25 | Anet Barrera Esparza (MEX) | Swapit Agolico | s.t. |
| 26 | Maria José Vargas Barrientos (CRC) | Swapit Agolico | s.t. |
| 27 | Susan Camacho Fallas (CRC) | | s.t. |
| 28 | Kennia Damaris Vargas Umaña (CRC) | | s.t. |
| 29 | Cintia Eugenia Lee Lopez (GUA) | | s.t. |
| 30 |  Paola Andrea Muñoz Grandon (CHI) | Swapit Agolico | 0:17 |
| 31 | Lady Johana Sepulveda Duran (COL) | | 0:17 |
| 32 | Yadira Torres Ruiz (PUR) | | 0:37 |
| 33 | Claudia Angelica Roncancio Cabra (COL) | | 0:37 |
| 34 | Cristina Torres Santini (PUR) | | 0:37 |
| 35 | Karen Elisa Amaya (HON) | | 0:37 |
| 36 | Stephany Castillo Malouf (GUA) | | 0:49 |
| 37 | Linda Daniela Menendez (HON) | | 0:56 |
| 38 | Marcia Fiorella Rojas Hernandez (CRC) | | 1:22 |
| 39 |  Mary Elizabeth Maroon (USA) | | 1:22 |
| 40 | Luisa Fernanda Naranjo Henao (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | 1:29 |
| 41 | Stephanie Nathalie Subercaseaux Vergara (CHI) | | 1:44 |
| 42 | Cristine De Mezerville Ferreto (CRC) | | 1:54 |
| 43 | Priscilla Chevez Gonzalez (CRC) | | 2:35 |
| 44 | Donelys Cariño Rivera (PUR) | | 2:46 |
| 45 | Diana Solano Rojas (CRC) | | 3:22 |
| 46 | Susana Patricia Santos Santos (GUA) | | 3:40 |
| 47 | Laura Sofia Castillo Murillo (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | 3:51 |
| 48 | Janet González (COL) | | 4:04 |
| 49 | Deborah Vega Gómez (PUR) | | 4:13 |
| 50 | Samantha Quijano (USA) | | 7:07 |
| 51 | Laura Lopez Torres (CRC) | | 8:34 |
| 52 | María Morales (GUA) | | 8:34 |
| 53 | Maria Andre Girón de León (GUA) | | 11:34 |
| 54 | Leydy Xiomara Buitrago Perdomo (COL) | | 12:58 |
| 55 |  Xenia Tomasa Estrada Villalta (SAL) | | 14:10 |
| 56 | Daniela Irais Gonzalez Garcia (MEX) | | 19:00 |
| 57 | Karen Canales (HON) | | 19:28 |
| 58 | Casandra Surillo (PUR) | | 34:16 |
| 59 | Maria Dolores Molina (GUA) | | |
| 60 | Evelyn Hound (USA) | | |
| 61 | Vi Tran (USA) | | |
| 62 |  Lilibeth de Carmen Chacón Garcia (VEN) | S.C. Michela Fanini Record Rox | |
| 63 | Maria Victoria Gutierrez del Aguila (GUA) | | |
