| 1 |  Silke Smulders (NED) | Liv-Alula-Jayco | 3:36:19 |
| 2 |  Margarita Victoria García Cañellas (SPA) | Liv-Alula-Jayco | s.t. |
| 3 |  Ella Wyllie (NZL) | Liv-Alula-Jayco | 1:29 |
| 4 |  Edseth Marte Berg (NOR) | Uno-X Mobility | 2:52 |
| 5 | Usoa Ostolaza Zabala (SPA) | Laboral Kutxa - Fundación Euskadi | 3:53 |
| 6 | Henrietta Christie (NZL) | Human Powered Health | s.t. |
| 7 | Mireia Benito Pellicer (SPA) | AG Insurance - Soudal Team | s.t. |
| 8 |  Simone Boilard (CAN) | Uno-X Mobility | s.t. |
| 9 | Mie Bjørndal Ottestad (NOR) | Uno-X Mobility | 4:00 |
| 10 |  Carlotta Cipressi (ITA) | | 4:05 |
| 11 | Katrine Aalerud (NOR) | Uno-X Mobility | 4:32 |
| 12 | Anouska Koster (NED) | Uno-X Mobility | 5:16 |
| 13 | Floortje Mackaij (NED) | | s.t. |
| 14 |  Alena Amialyusik (BLR) | Uae Team Adq | 5:24 |
| 15 |  Yuliia Biriukova (UKR) | Human Powered Health | 7:21 |
| 16 |  Paula Andrea Patiño Bedoya (COL) | Movistar Team | 7:22 |
| 17 | Iurani Blanco Calbet (SPA) | Laboral Kutxa - Fundación Euskadi | s.t. |
| 18 |  Justyna Czapla (GER) | Canyon - Sram Racing | 7:28 |
| 19 | Giorgia Vettorello (ITA) | Roland | 7:31 |
| 20 | Selene Colombi (ITA) | | 10:54 |
| 21 | Quinty Ton (NED) | Liv-Alula-Jayco | 11:14 |
| 22 |  Rasa Leleivyte (LTU) | Aromitalia 3T Vaiano | 13:45 |
| 23 |  Miryan Maritza Nuñez Padilla (ECU) | Primeau Vélo - Groupe Abadie | 13:49 |
| 24 |  Inna Abaidullina (RUS) | | s.t. |
| 25 |  Ariadna Gutierrez Arzaluz (MEX) | | s.t. |
| 26 |  Joscelin Lowden (GBR) | Uno-X Mobility | s.t. |
| 27 | Leonie Laubig (GER) | Primeau Vélo - Groupe Abadie | 13:54 |
| 28 | Isabella Bertold (CAN) | | s.t. |
| 29 | Federica Damiana Piergiovanni (ITA) | | s.t. |
| 30 | Helena Moragas Molina (SPA) | | 13:59 |
| 31 | Eneritz Vadillo Crespo (SPA) | Laboral Kutxa - Fundación Euskadi | 14:01 |
| 32 |  Arlenis Sierra Cañadilla (CUB) | Movistar Team | s.t. |
| 33 | Nadia Quagliotto (ITA) | Laboral Kutxa - Fundación Euskadi | s.t. |
| 34 | Elizabeth Holden (GBR) | Uae Team Adq | s.t. |
| 35 | Cristina Tonetti (ITA) | Laboral Kutxa - Fundación Euskadi | s.t. |
| 36 | Sheyla Gutiérrez Ruiz (SPA) | Movistar Team | s.t. |
| 37 |  Jelena Eric (SCG) | Movistar Team | 14:21 |
| 38 | Iris Gómez Argiles (SPA) | | 17:24 |
| 39 |  Ana Caramelo (POR) | | 18:40 |
| 40 |  Nathalie Eklund (SWE) | Roland | 19:19 |
| 41 |  Alexandra Manly (AUS) | Liv-Alula-Jayco | s.t. |
| 42 | Daniela Campos (POR) | Eneicat - Cmteam | s.t. |
| 43 | Ariana Gilabert Vilaplana (SPA) | Eneicat - Cmteam | s.t. |
| 44 | Lourdes Oyarbide Jimenez (SPA) | Laboral Kutxa - Fundación Euskadi | s.t. |
| 45 | Ingrid Ruiz Sanchez (SPA) | | s.t. |
| 46 | Marit Raaijmakers (NED) | Human Powered Health | s.t. |
| 47 | Sylvie Swinkels (NED) | Roland | 19:22 |
| 48 |  Linda Zanetti (SWI) | Human Powered Health | s.t. |
| 49 |  Kristabel Doebel-Hickok (USA) | Human Powered Health | s.t. |
| 50 | Fanny Bonini (ITA) | Aromitalia 3T Vaiano | 19:36 |
| 51 | Natalia Ovejero López (SPA) | | 19:38 |
| 52 | Mireia Trias Jordán (SPA) | | 26:35 |
| 53 | Ainhoa Moreno Ossowsky (SPA) | Aromitalia 3T Vaiano | s.t. |
| 54 |  Agua Marina Espínola Salinas (PAR) | Primeau Vélo - Groupe Abadie | s.t. |
| 55 |  Alice Coutinho (FRA) | | s.t. |
| 56 | Nicolien Luijsterburg (NED) | | 28:45 |
| 57 | Andrea Álzate Gomez (COL) | Eneicat - Cmteam | 31:38 |
| 58 | Nahia Imaz Perez (SPA) | | s.t. |
| 59 | Awen Roberts (GBR) | | 33:59 |
| 60 | Mathilde Huot (CAN) | Primeau Vélo - Groupe Abadie | 35:13 |
| 61 | Kateryna Velychko (UKR) | | s.t. |
| 62 |  Agustina Reyes Perdomo (URU) | | 35:15 |
| 63 | Yuliia Pchelintseva (UKR) | | s.t. |
| 64 |  Ana Vitória Gouvea Vieira Almeida Magalhães (BRA) | | s.t. |
| 65 | Andrea Martinez Lopez (MEX) | | s.t. |
| 66 | Prisca Savi (ITA) | | s.t. |
| 67 | Beatrice Pozzobon (ITA) | | s.t. |
| 68 | Valentina Basilico (ITA) | Eneicat - Cmteam | s.t. |
| 69 | Francesca Pellegrini (ITA) | | s.t. |
| 70 | Marina Garau Roca (SPA) | | 35:17 |
| 71 | Irene Affolati (ITA) | Aromitalia 3T Vaiano | 35:22 |
| 72 | Andrea Perez Figueroa (SPA) | | 35:28 |
| 73 | Marta Romance I Oms (SPA) | | s.t. |
| 74 |  Luciana Roland (ARG) | | s.t. |
| 75 | Maggie Coles-Lyster (CAN) | Roland | s.t. |
| 76 | Ainara Albert Bosch (SPA) | | 35:41 |
| 77 | Diana Pedrosa (POR) | | 35:43 |
| 78 | Romina Costantini (ITA) | | 36:06 |
| 79 | Maria Banlles Santamaria (SPA) | | 39:56 |
| 80 |  Diane Ingabire (RWA) | | s.t. |
| 81 | Jule Märkl (GER) | | 47:26 |
| 82 | Cybèle Schneider (SWI) | | s.t. |
| 83 | Anabelle Thomas (CAN) | Primeau Vélo - Groupe Abadie | 52:04 |
| 84 |  Ese Lovina Ukpeseraye (NGR) | | 52:15 |
| 85 | Isabella Maria Escalera (SPA) | | s.t. |
| 86 | Lara Scarselli (ITA) | Aromitalia 3T Vaiano | s.t. |
| 87 | Laura Rodriguez Cordero (SPA) | | s.t. |
| 88 | Almudena Morales Perez (SPA) | | s.t. |
| 89 | Carolina Vega (SPA) | | 53:18 |
| 90 | Ziortza Isasi Cristobal (SPA) | | |
| 91 | Eva Anguela Yaguez (SPA) | Primeau Vélo - Groupe Abadie | |
| 92 |  Daniela Schmidsberger (AUT) | | |
| 93 | Lola Bryson-Boe (NZL) | | |
| 94 |  Tusnelda Svanholmer (DEN) | | |
| 95 | Odette Lynch (AUS) | | |
| 96 | Cristina Garcia Pulido (SPA) | | |
| 97 | Marta Carvalho (POR) | | |
| 98 | Tamara Dronova-Balabolina (RUS) | Roland | |
