| 1 |  Cecilie Uttrup Ludwig (DEN) | | 2:36:21 |
| 2 |  Marta Cavalli (ITA) | Fdj - Suez | 0:05 |
| 3 |  Juliette Labous (FRA) | Team Dsm | 0:16 |
| 4 | Gaia Realini (ITA) | Trek - Segafredo | 0:28 |
| 5 |  Ane Santesteban González (SPA) | Groupama - Fdj | 0:32 |
| 6 |  Liane Lippert (GER) | Movistar Team | 0:38 |
| 7 | Sofia Bertizzolo (ITA) | Uae Team Adq | 0:47 |
| 8 | Évita Muzic (FRA) | Fdj - Suez | 0:50 |
| 9 | Erica Magnaldi (ITA) | Uae Team Adq | 1:12 |
| 10 |  Urska Zigart (SLO) | Groupama - Fdj | 1:20 |
| 11 | Silvia Persico (ITA) | Uae Team Adq | s.t. |
| 12 |  Christina Schweinberger (AUT) | | 1:33 |
| 13 | Clara Koppenburg (GER) | Cofidis Women Team | 1:57 |
| 14 | Laura Asencio (FRA) | | s.t. |
| 15 |  Loes Adegeest (NED) | Fdj - Suez | 2:02 |
| 16 | Francesca Barale (ITA) | Team Dsm | s.t. |
| 17 | Nienke Vinke (NED) | Team Dsm | s.t. |
| 18 | Beatrice Rossato (ITA) | Isolmant - Premac - Vittoria | 2:04 |
| 19 |  Ingvild Gåskjenn (NOR) | Groupama - Fdj | 2:07 |
| 20 | Esmée Peperkamp (NED) | Team Dsm | s.t. |
| 21 | Ursa Pintar (SLO) | Btc - City Ljubljana - Scott | 2:15 |
| 22 |  Alena Amialyusik (BLR) | Uae Team Adq | s.t. |
| 23 |  Paula Andrea Patiño Bedoya (COL) | Movistar Team | 2:20 |
| 24 | Cristina Tonetti (ITA) | | 2:23 |
| 25 | Nina Buijsman (NED) | Human Powered Health | 2:24 |
| 26 | Anna Kiesenhofer (AUT) | Team Jayco Alula | 2:28 |
| 27 | Giorgia Vettorello (ITA) | Be Pink | 2:30 |
| 28 | Julie Bego (FRA) | | 2:33 |
| 29 |  Rasa Leleivyte (LTU) | Aromitalia - Basso Bikes - Vaiano | s.t. |
| 30 | Greta Marturano (ITA) | Fenix-Deceuninck | 2:37 |
| 31 | Anne Knijnenburg (NED) | | 2:39 |
| 32 | Elisa Valtulini (ITA) | | s.t. |
| 33 | Elena Pirrone (ITA) | Team Jayco Alula | 2:55 |
| 34 | Marta Romeu Solaz (SPA) | Laboral Kutxa - Fundación Euskadi | 2:56 |
| 35 | Hannah Ludwig (GER) | Uno-X Pro Cycling Team | s.t. |
| 36 | Barbara Malcotti (ITA) | Human Powered Health | 2:57 |
| 37 | Natalia Franco Villegas (COL) | | 2:58 |
| 38 |  Hanna Nilsson (SWE) | Ceratizit - Wnt | 3:07 |
| 39 |  Claire Steels (GBR) | Team Jayco Alula | 3:14 |
| 40 |  Arlenis Sierra Cañadilla (CUB) | Movistar Team | 3:24 |
| 41 |  Jelena Eric (SCG) | Movistar Team | s.t. |
| 42 | Arianna Fidanza (ITA) | Ceratizit - Wnt | 3:33 |
| 43 | Edseth Marte Berg (NOR) | Uno-X Pro Cycling Team | s.t. |
| 44 | Alessia Vigilia (ITA) | | 3:38 |
| 45 | Jade Wiel (FRA) | Fdj - Suez | 3:47 |
| 46 |  Emilie Fortin (CAN) | | 3:49 |
| 47 | Anouska Koster (NED) | Uno-X Pro Cycling Team | 3:57 |
| 48 | Silvia Zanardi (ITA) | Be Pink | 3:59 |
| 49 | Rosita Reijnhout (NED) | Team Jumbo - Visma | 4:07 |
| 50 | Usoa Ostolaza Zabala (SPA) | | s.t. |
| 51 | Debora Silvestri (ITA) | | 4:11 |
| 52 | Eneritz Vadillo Crespo (SPA) | Laboral Kutxa - Fundación Euskadi | 4:19 |
| 53 |  Tamara Dronova-Balabolina (RUS) | Team Jayco Alula | 4:20 |
| 54 | Marta Romance I Oms (SPA) | | 4:21 |
| 55 | Pauline Allin (FRA) | | s.t. |
| 56 |  Rachel Neylan (AUS) | Cofidis Women Team | 4:23 |
| 57 | Giulia Giuliani (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 4:34 |
| 58 | Letizia Paternoster (ITA) | Groupama - Fdj | 4:36 |
| 59 | Gaia Segato (ITA) | | 4:37 |
| 60 | Nika Bobnar (SLO) | Btc - City Ljubljana - Scott | s.t. |
| 61 | Sophie Wright (GBR) | Fenix-Deceuninck | 4:41 |
| 62 | Femke Gerritse (NED) | Parkhotel Valkenburg | 4:43 |
| 63 | Lieke Nooijen (NED) | Parkhotel Valkenburg | 4:44 |
| 64 | Matilde Vitillo (ITA) | Be Pink | 4:50 |
| 65 | Tjasa Susnik (SLO) | Btc - City Ljubljana - Scott | s.t. |
| 66 | Irene Affolati (ITA) | Aromitalia - Basso Bikes - Vaiano | 5:01 |
| 67 | Chiara Doni (ITA) | | s.t. |
| 68 | Maria Giulia Confalonieri (ITA) | Uno-X Pro Cycling Team | 5:16 |
| 69 | Léa Curinier (FRA) | Team Dsm | 5:18 |
| 70 | Elise Uijen (NED) | Team Dsm | s.t. |
| 71 | Morgane Coston (FRA) | Cofidis Women Team | s.t. |
| 72 | Angela Oro (ITA) | | s.t. |
| 73 | Monica Castagna (ITA) | | 5:20 |
| 74 | Yana Myalitsina (RUS) | | 5:29 |
| 75 |  Antri Christoforou (CYP) | Human Powered Health | 5:35 |
| 76 | Vittoria Guazzini (ITA) | Fdj - Suez | 5:43 |
| 77 | Chiara Consonni (ITA) | Uae Team Adq | s.t. |
| 78 |  Jarmila Machacová (CZE) | | s.t. |
| 79 | Nadia Quagliotto (ITA) | Laboral Kutxa - Fundación Euskadi | 5:47 |
| 80 | Chiara Reghini (ITA) | | s.t. |
| 81 | Virginia Bortoli (ITA) | | s.t. |
| 82 | Marit Raaijmakers (NED) | Human Powered Health | s.t. |
| 83 | Amber Pate (AUS) | Groupama - Fdj | 5:52 |
| 84 | Carmela Cipriani (ITA) | Isolmant - Premac - Vittoria | 5:53 |
| 85 | Andrea Casagranda (ITA) | Be Pink | 5:55 |
| 86 |  Selam Amha Gerefiel (ETH) | | 6:01 |
| 87 | Alice Maria Arzuffi (ITA) | Ceratizit - Wnt | 6:05 |
| 88 | Sara Martín Martín (SPA) | Movistar Team | 6:09 |
| 89 |  Nicole Steinmetz (USA) | | 6:12 |
| 90 | Serena Brillante Romeo (ITA) | | 6:14 |
| 91 | Valeria Curnis (ITA) | | 6:16 |
| 92 | Dziyana Lebedz (BLR) | | 6:44 |
| 93 |  Henrietta Christie (NZL) | Human Powered Health | 6:50 |
| 94 | Olivia Baril (CAN) | Uae Team Adq | 6:55 |
| 95 | Vittoria Ruffilli (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | 6:57 |
| 96 | Iris Monticolo (ITA) | | 7:00 |
| 97 | Sara Pepoli (ITA) | Isolmant - Premac - Vittoria | s.t. |
| 98 |  Imogen Cotter (IRL) | Fenix-Deceuninck Development Team | 7:13 |
| 99 | Evy Kuijpers (NED) | Fenix-Deceuninck | 7:20 |
| 100 | Giulia Luciani (ITA) | Born to Win G20 Ambedo | 7:24 |
| 101 |  Anna Kolyzhuk (UKR) | | 7:39 |
| 102 | Millie Couzens (GBR) | Fenix-Deceuninck | 7:40 |
| 103 | Elinor Barker (GBR) | Uno-X Pro Cycling Team | s.t. |
| 104 | Kirstie van Haaften (NED) | Parkhotel Valkenburg | 7:58 |
| 105 | Asia Zontone (ITA) | Isolmant - Premac - Vittoria | 8:04 |
| 106 | Barbora Nemcová (CZE) | | 8:07 |
| DNF | Elisa Balsamo (ITA) | Trek - Segafredo | - |
| DNF | Elynor Backstedt (GBR) | Trek - Segafredo | - |
| DNF | Ilaria Sanguineti (ITA) | Trek - Segafredo | - |
| DNF | Nathalie Eklund (SWE) | Team Jayco Alula | - |
| DNF | Elizabeth Stannard (AUS) | | - |
| DNF | Mareille Meijering (NED) | | - |
| DNF | Amber Kraak (NED) | Team Jumbo - Visma | - |
| DNF | Linda Riedmann (GER) | Team Jumbo - Visma | - |
| DNF | Maud Oudeman (NED) | Team Jumbo - Visma | - |
| DNF | Eva Van Agt (NED) | Team Jumbo - Visma | - |
| DNF | Wilma Olausson (SWE) | Uno-X Pro Cycling Team | - |
| DNF | Francesca Balducci (ITA) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Milena Del Sarto (ITA) | Aromitalia - Basso Bikes - Vaiano | - |
| DNF | Fanny Bonini (ITA) | | - |
| DNF | Lara Crestanello (ITA) | Be Pink | - |
| DNF | Vittoria Grassi (ITA) | | - |
| DNF | Rachele Bonzanini (ITA) | Born to Win G20 Ambedo | - |
| DNF | Sara Casasola (ITA) | Born to Win G20 Ambedo | - |
| DNF | Nika Myalitsina (RUS) | | - |
| DNF | Hana Zumer (SLO) | Btc - City Ljubljana - Scott | - |
| DNF | Spela Colnar (SLO) | Btc - City Ljubljana - Scott | - |
| DNF | Ema Pirs (SLO) | Btc - City Ljubljana - Scott | - |
| DNF | Martina Fidanza (ITA) | Ceratizit - Wnt | - |
| DNF | Lana Eberle (GER) | Ceratizit - Wnt | - |
| DNF | Flavie Boulais (FRA) | Cofidis Women Team | - |
| DNF | Tess Lawson (GBR) | | - |
| DNF | Gemma Sernissi (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Noemi Lucrezia Eremita (ITA) | Isolmant - Premac - Vittoria | - |
| DNF | Ashley Frye (USA) | | - |
| DNF | Greta Richioud (FRA) | | - |
| DNF | Sonia Rossetti (ITA) | GB Junior Team Piemonte Pedale Castanese A.S.D. | - |
| DNF | Mireia Trias Jordán (SPA) | Laboral Kutxa - Fundación Euskadi | - |
| DNF | Quinty Schoens (NED) | Parkhotel Valkenburg | - |
| DNF | Scarlett Souren (NED) | Parkhotel Valkenburg | - |
| DNF | Veronika Bartoníková (CZE) | | - |
| DNF | Gabriela Bártová (CZE) | | - |
| DNF | Nicole Hartychová (CZE) | | - |
| DNF | Nela Slaníková (CZE) | | - |
| DNF | Francesca Pisciali (ITA) | | - |
| DNF | Elisa Lugli (ITA) | | - |
| DNF | Alice Capasso (ITA) | | - |
| DNF |  Luciana Roland (ARG) | | - |
| DNF |  Elina Tasane (EST) | | - |
